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KMID : 0379120000280040171
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Korean Journal of Mycology
2000 Volume.28 No. 4 p.171 ~ p.176
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Genomic Organization of ancop Gene for a - COP Homolog from Aspergillus nidulans
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Park, Hee Moon
Maeng, Pil Jae/Kim, Jeong Yoon/Lee, Hwan Hee/Chae, Shun Kee
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Abstract
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We have cloned a α-COP homolog, ancop, from Aspergillus nidulans by rnlony hybridization of chromosome specific library using α-COP homologous fragment as a probe. The probe DNA was amplified with degenerated primers designed by comparison of conserved ton of the amino acid sequences of Saccharomyces cerevisiae α-COP, Homo Sapiens HEP-COP, and Drosophila melanogaster α-COP. Full length cDNA clone was also amplified by RT-PCR. Comparison of genomic DNA sequence with cDNA sequence obtained by RT-PCR revealed 7 introns. Amino acid sequence similarity search of the anCop with other α-COPS gave an overall identity of 52% with S. cerevisiae, 47% with human and bovine, 45% with Drosophila and Arabidopsu. In upstream region from the transcription start site, a putative TATA and CAAT motif were also identified.
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